ImageXpress Confocal HT.aiIntelligent confocal high connotation imaging analysis systemA 7-color laser light source with 8 imaging channels was used to achieve highly scalable multi-channel imaging analysis, while maintaining high throughput by shortening exposure time.The water mirror system improves image resolution and reduces aberrations, allowing scientists to see thick samples more deeply.
MetaXpress software and IN Carta ™ The powerful combination of software simplifies the workflow of advanced phenotype classification and 3D image analysis, with machine learning capabilities and intuitive user interface.

main features

•8-channel 7-color laser light sourceCompared to LED light sources, it can generate brighter images and higher intensity signals, while doubling the acquisition speed for most 3D organoid and sphere analyses.
• Rotary table confocal technologyReduce the interference caused by defocused light, allowing tissues to penetrate deeper, producing clearer images, and improving axial resolution.
Automated water mirror technologyProvide up to 4x signal enhancement without sacrificing speed, achieving greater sensitivity and image clarity.
• IN Carta softwareUtilizing modern machine learning technology to achieve easy to operate, workflow guided high content image analysis.

AccurateAssist in exploring more possibilities
• AgileOptix ™ Rotary confocal technology eliminates the interference of defocused light and provides a deeper understanding of thick tissue samples
Machine learning reduces classification errors and enhances high-throughput screening and analysis of complex models
• Quickly image and identify cells and intracellular events
Unbiased cell segmentation and phenotype feature extraction

"Accurate”Assist in exploring more possibilities
AgileOptix ™ Rotary confocal technology is a unique optical system of MD Company, which allows for easy switching and setting of shooting modes to achieve better shooting and analysis results.
ImageXpress Confocal HT.aiAdopting AgileOptix technology. In the confocal configuration options, there are multiple selectable confocal turntables and 7 laser excitation channels, making configuration selection more convenient and enabling diverse and flexible configurations for unique experimental needs. The intelligent design of optical devices with high-power lasers and sCMOS detectors enhances sensitivity.

8 imaging channels
• DAPI • CFP • FITC • YFP
• TRITC • Texas Red • Cy5 • Cy7

Support imaging analysis from subcellular to whole tissue level
• Wide sample applicability (over 25 objective lenses to choose from)
The numerical aperture of the oil mirror can reach 1.4
The numerical aperture of the air mirror can be selected from 0.05 to 0.95 (1X to 100X objective lenses can be configured)
The numerical aperture of the water mirror can reach 1.2 (can be configured with 20X, 40X, and 60X objective lenses)

Choose a turntable confocal module that is more suitable for experimental needs

*SCMOS ensures the implementation of performance

performance

Flexible AssistanceExplore more possibilities
Using 3D cultivation models to obtain data closer to in vivo experiments
QuickID Targeted Image Acquisition
QuickID sorts objects or rare events of interest under low magnification and automatically images them under high magnification. It can flexibly obtain images of various formats and sizes, and can be scaled according to constantly changing research needs.

The process of sphere image acquisition was implemented using QuickID.The image obtained under low magnification can be
Observe the entire hole within one field of view for target recognition, and use Z-axis multi-layer scanning under higher magnification
The 3-color fluorescence channel automatically re images.

Complex 3D cultured cell models are increasingly being used in drug development and basic research, mainly because compared to single-layer and 2D cultured models, 3D models are closer to the in vivo environment and can obtain more predictive and physiologically meaningful results. The ImageXpress Confocal HT.ai system provides a simple and fast method for detecting 3D models, and even samples grown in thick Matrigel can achieve better results. The system provides a flexible imaging experience and multiple configuration options, such as laser light sources and water mirror systems, which can improve sample detection depth and eliminate optical aberrations.

Clearer sample images and more accurate data
• 3D cell spheres • Thick tissue slice samples • Organoids • Cell staining • Zebrafish and nematodes • Homogeneous wash free experiments

Staining of cell nuclei and skeletons cultured in 3D gel.40X flat field complex
Achromatic objective lens shooting, Z-axis 7-layer projection processed image.

High throughput long-term dynamic testing of integrity systems
The ImageXpress Confocal HT.ai system can quickly and conveniently expand the experimental scale of 3D drug discovery. By utilizing transmitted light and various fluorescent cell markers, this system can be used for accurate monitoring of organoid growth and cell dynamics. Humidity CO2 The environmental control module for level and temperature regulation can maintain the vitality of cells for delayed experiments ranging from a few minutes to several days.

Image of cell spheres in a U-shaped circular bottom microplate.Experimental screening of compound dose-response curves based on concentration effects. One field of view can cover the entire cell sphere,

After scanning the entire plate, assemble the 384 well plate into a puzzle to obtain a large image of the entire plate. The right figure shows the concentration dependent curve of the number of live cells.

Efficient AssistanceExplore more possibilities

The seamless workflow provides a complete solution for screening highly complex biological problems
The intelligent co focus high connotation system has a complete solution for integrating environmental control, which can explore complex biological problems with simple operation processes.

Acquire Image
The powerful control functions of MetaXpress high content image acquisition and analysis software enable all image acquisition and data analysis tasks to be completed in the same interface.
Complete focusing method (laser+image automatic focusing), which can achieve the focusing of the entire sample
Long term imaging of live cells can detect various biological phenomena such as cell proliferation, death, differentiation, and migration, virus and bacterial infection, tumor cell metastasis, chemotaxis, drug toxicity, translocation, etc

data analysis
Whether you need routine analysis or customized analysis, MetaXpress can meet your needs for rapid data analysis.
One click operation of preset modules, easy to get started, can achieve hundreds of experimental analyses
User customized editing module, flexible application, including multiple filters and algorithms, can produce your own analysis methods
Adaptive Background CorrectionTM）Background can be removed based on the fluorescence intensity values of adjacent backgrounds, and target samples can be segmented to achieve better segmentation results
2D projection imaging, including best focus, maximum, minimum, and brightness overlay projection, enables easy analysis of 3D images
3D volume analysis evaluation, XYZ position, distance to adjacent objects, diameter, depth, various fluorescence intensity measurements, texture or number of objects
All analysis parameters can obtain corresponding values for each cell or the average value for each field of view separately

Convenient AssistanceExplore more possibilities

Powerful analysis combined with intuitive user interface and machine learning capabilities simplifies the workflow of high-order phenotype classification and 3D image analysis

IN Carta image analysis software
The IN Carta software makes it easier for users to accept the complexity of images. By combining powerful analysis capabilities and modern user interfaces, the process of obtaining biological insights from 2D, 3D, and time-lapse data becomes more efficient. Machine learning techniques and guided workflows create a better user experience, making higher-order phenotype analysis more intuitive and results more reliable. No need for image analysis experts or lengthy adjustments and testing of experimental parameters. The IN Carta software takes on the heavy workload, allowing scientists to focus on their research.
Guided workflows and scalable batch processing capabilities enhance work efficiency. No need to waste time on tedious analysis settings
The optimized calculation mode provides unbiased results quickly through visualization
Machine learning technology utilizes more information to reduce errors in high content screening data analysis, helping researchers explore more new discoveries
Intuitive user experience and cutting-edge technology minimize the software learning curve, eliminating barriers to efficient and convenient image processing and analysis

The scatter plot data in IN Carta software displays the distribution of kernel area (x-axis) and kernel diameter (y-axis).

The segmentation mask represents the nucleus (blue), endoplasmic reticulum (green outline), and actin filaments (magenta)
Outline).